Double labeling with iodo- and bromodeoxyuridine for cell kinetics studies.
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منابع مشابه
Double Labeling of S-Phase Murine Cells with Bromodeoxyuridine and a Second DMA-specific Probe1
A rapid method has been developed which combines immunofluorescence and autoradiography and permits the double labeling of DNA. P388 murine leukemic cells were incubated with bromodeoxyuridine and tritiated thymidine simultaneously. After fixation, the sample was first processed with a monoclonal antibody to bromodeoxyuridine (RPMB I) so that any cell in Sphase was brightly fluorescent (RPMB te...
متن کاملDouble labeling of S-phase murine cells with bromodeoxyuridine and a second DNA-specific probe.
A rapid method has been developed which combines immunofluorescence and autoradiography and permits the double labeling of DNA. P388 murine leukemic cells were incubated with bromodeoxyuridine and tritiated thymidine simultaneously. After fixation, the sample was first processed with a monoclonal antibody to bromodeoxyuridine (RPMB I) so that any cell in S-phase was brightly fluorescent (RPMB t...
متن کاملCell migration and aggregation in the developing telencephalon: pulse-labeling chick embryos with bromodeoxyuridine.
Previous studies had concluded that the avian telencephalon develops according to an outside-in schedule of neurogenesis, with relatively little migration of young neuroblasts past older cells. These previous studies had, however, been based on the "cumulative labeling" method, which is less accurate than the "pulse-labeling" method typically used in mammals. In the present study, we pulse-labe...
متن کاملCell Kinetics in Human Malignancies Studied with in Vivo Administration of Bromodeoxyuridine and Flow Cytometry1
Bromodeoxyuridine (BrdUrd) is a pyrimidine analogue which is incor porated into the DN A of proliferating cells. When in vivoBrdUrd infusion is coupled with Divariate flow cytomctry to measure cell BrdUrd incor poration and I)V\ content, both the percentage of DNA-synthesizing cells jBrdUrd-labeling index (LI)] and the DNA synthesis time (Ts) can be determined on the same tissue sample. From ex...
متن کاملCell kinetics in human malignancies studied with in vivo administration of bromodeoxyuridine and flow cytometry.
Bromodeoxyuridine (BrdUrd) is a pyrimidine analogue which is incorporated into the DNA of proliferating cells. When in vivo BrdUrd infusion is coupled with bivariate flow cytometry to measure cell BrdUrd incorporation and DNA content, both the percentage of DNA-synthesizing cells [BrdUrd-labeling index (LI)] and the DNA synthesis time (TS) can be determined on the same tissue sample. From exper...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1989
ISSN: 0022-1554,1551-5044
DOI: 10.1177/37.7.2659659